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利用邻近结扎测定法在人类胰腺β细胞中原位内源性米托巴结物的可视化
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Medicine
Visualization of Endogenous Mitophagy Complexes In Situ in Human Pancreatic Beta Cells Utilizing Proximity Ligation Assay
Please note that all translations are automatically generated.
Click here for the English version.
利用邻近结扎测定法在人类胰腺β细胞中原位内源性米托巴结物的可视化
DOI:
10.3791/59398-v
•
08:40 min
•
May 02, 2019
•
Gemma Pearson
,
Scott A. Soleimanpour
2
1
Department of Internal Medicine, Division of Metabolism, Endocrinology and Diabetes
,
University of Michigan, Ann Arbor
,
2
VA Ann Arbor Healthcare System
Chapters
00:04
Title
00:32
Human Islet Sample Single Cell Dissociation
02:14
Single Cell Adherence and Fixation and Immunohistochemistry
03:24
Proximity Ligation Assay (PLA)
04:38
Imaging
06:00
PLA Interaction Quantification
07:18
Results: Representative Beta Cell Mitophagy Analysis
08:08
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议概述了一种定量分析从原人类小岛样本的β细胞中专门形成米托巴细胞蛋白复合体的方法。因此,该技术允许从有限的生物材料分析米托巴,这在珍贵的人类胰腺β细胞样本中至关重要。
Tags
Mitophagy
Proximity Ligation Assay
Human Pancreatic Beta Cells
Endogenous Mitophagy Complexes
Immunohistochemistry
Ubiquitin-specific Peptidase 8
Neuregulin Receptor Degradation Protein-1
Beta Cell Marker
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