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精密切肺切片作为离体肺血管结构和收缩力研究的有效工具

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JoVE Journal Medicine

Precision Cut Lung Slices as an Efficient Tool for Ex vivo Pulmonary Vessel Structure and Contractility Studies

Please note that all translations are automatically generated. Click here for the English version.

精密切肺切片作为离体肺血管结构和收缩力研究的有效工具

DOI:

10.3791/62392-v

•

09:08 min

•

May 24, 2021

•

Timothy Klouda, Hyunbum Kim, Jiwon Kim, Gary Visner, Ke Yuan

¹Divisions of Pulmonary Medicine,Boston Children’s Hospital

Chapters

00:04Introduction
00:49Extraction of Mouse Lung
03:34Precision Cut Lung Slices (PCLS)
05:35Vasoconstrictor Experiment
06:20Preparing the Tissue for RNA or Protein Lysis on PCLS
07:26Results: Ex Vivo Pulmonary Vessel Structure and Contractility Studies of PCLS
08:32Conclusion

Summary

Automatic Translation

这里介绍的是用于保存PCLS小鼠肺组织的血管收缩力的方案，从而产生肺脉管系统和气道的复杂三维图像，可以保存长达10天，容易受到许多程序的影响。

Tags

Precision Cut Lung Slices Ex Vivo Pulmonary Vessel Contractility Structure Spatial Orientation Live Surrogate Model Signaling Cascades Pulmonary Vascular Diseases Euthanasia Trachea Right Ventricle Heparin PBS Agarose Vibratome DMEM

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