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小鼠间质瓣细胞分离研究主动脉瓣体外钙化
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JoVE Journal Biology
Isolation of Mouse Interstitial Valve Cells to Study the Calcification of the Aortic Valve In Vitro
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小鼠间质瓣细胞分离研究主动脉瓣体外钙化

DOI:
10.3791/62419-v

•

05:47 min

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May 10, 2021

•

,
1Cardiovascular Research Center,The Icahn School of Medicine at Mount Sinai, 2Diabetes, Obesity and Metabolism Institute, Department of Medicine,The Icahn School of Medicine at Mount Sinai, 3Graduate School of Biomedical Sciences,The Icahn School of Medicine at Mount Sinai

Chapters

  • 00:04Introduction
  • 00:43Isolation of Valve Cells
  • 03:35In vitro Calcification Assay
  • 04:23Results: Identification of Mouse Valve Cell Phenotype and Calcification Assay
  • 05:02Conclusion

Summary

Automatic Translation

Automatic Translation

本文描述了通过两步拼贴程序分离小鼠主动脉瓣细胞。分离的小鼠瓣细胞对于执行不同的测定（如 体外 钙化检测）和研究导致主动脉瓣矿化的分子通路非常重要。

Tags

Mouse Interstitial Valve CellsAortic Valve CalcificationIn VitroTwo-step DigestionAortic Valve IsolationCollagenaseHEPESCell CultureCell SeparationCentrifugation

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