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Biology
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小鼠间质瓣细胞分离研究主动脉瓣
体外
钙化
JoVE Journal
Biology
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JoVE Journal
Biology
Isolation of Mouse Interstitial Valve Cells to Study the Calcification of the Aortic Valve
In Vitro
Please note that all translations are automatically generated.
Click here for the English version.
小鼠间质瓣细胞分离研究主动脉瓣
体外
钙化
DOI:
10.3791/62419-v
•
05:47 min
•
May 10, 2021
•
Rihab Bouchareb
,
Djamel Lebeche
2,3
1
Cardiovascular Research Center
,
The Icahn School of Medicine at Mount Sinai
,
2
Diabetes, Obesity and Metabolism Institute, Department of Medicine
,
The Icahn School of Medicine at Mount Sinai
,
3
Graduate School of Biomedical Sciences
,
The Icahn School of Medicine at Mount Sinai
Chapters
00:04
Introduction
00:43
Isolation of Valve Cells
03:35
In vitro Calcification Assay
04:23
Results: Identification of Mouse Valve Cell Phenotype and Calcification Assay
05:02
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文描述了通过两步拼贴程序分离小鼠主动脉瓣细胞。分离的小鼠瓣细胞对于执行不同的测定(如
体外
钙化检测)和研究导致主动脉瓣矿化的分子通路非常重要。
Tags
Mouse Interstitial Valve Cells
Aortic Valve Calcification
In Vitro
Two-step Digestion
Aortic Valve Isolation
Collagenase
HEPES
Cell Culture
Cell Separation
Centrifugation
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