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细胞外囊泡的量化和尺寸测定纳米粒子跟踪分析
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Biology
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JoVE Journal
Biology
Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles
Please note that all translations are automatically generated.
Click here for the English version.
细胞外囊泡的量化和尺寸测定纳米粒子跟踪分析
DOI:
10.3791/62447-v
•
09:19 min
•
March 28, 2021
•
Nicole Comfort
,
Kunheng Cai
,
Tessa R. Bloomquist
,
Madeleine D. Strait
,
Anthony W. Ferrante Jr.
,
Andrea A. Baccarelli
1
Department of Environmental Health Sciences
,
Columbia University Mailman School of Public Health
,
2
Department of Medicine
,
Naomi Berrie Diabetes Center, Columbia University
Chapters
00:04
Introduction
00:58
Cuvette Preparation
01:53
Particle Tracking Instrument Preparation and Nanoparticle Tracking Analysis
04:01
NTA Data Processing, Display, and Interpretation
05:29
Cuvette Cleaning
06:33
Results: Using a Novel NTA Instrument to Estimate EV Size Distribution and Total Particle Concentration
08:33
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们演示了如何使用新型纳米粒子跟踪分析仪器来估计从小鼠围产层组织和人类血浆中分离出来的细胞外囊泡的大小分布和总颗粒浓度。
Tags
Nanoparticle Tracking Analysis
Extracellular Vesicles
NTA Parameters
Sample Preparation
Cuvette
Particle Concentration
Dilution
Focus
Frame Rate
Exposure Time
Stir Time
Reproducibility
Standardization
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