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细胞外囊泡的量化和尺寸测定纳米粒子跟踪分析

JoVE Journal
Biology

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JoVE Journal Biology

Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles

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细胞外囊泡的量化和尺寸测定纳米粒子跟踪分析

DOI:

10.3791/62447-v

•

09:19 min

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March 28, 2021

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Nicole Comfort, Kunheng Cai, Tessa R. Bloomquist, Madeleine D. Strait, Anthony W. Ferrante Jr., Andrea A. Baccarelli

¹Department of Environmental Health Sciences,Columbia University Mailman School of Public Health, ²Department of Medicine,Naomi Berrie Diabetes Center, Columbia University

Chapters

00:04Introduction
00:58Cuvette Preparation
01:53Particle Tracking Instrument Preparation and Nanoparticle Tracking Analysis
04:01NTA Data Processing, Display, and Interpretation
05:29Cuvette Cleaning
06:33Results: Using a Novel NTA Instrument to Estimate EV Size Distribution and Total Particle Concentration
08:33Conclusion

Summary

Automatic Translation

我们演示了如何使用新型纳米粒子跟踪分析仪器来估计从小鼠围产层组织和人类血浆中分离出来的细胞外囊泡的大小分布和总颗粒浓度。

Tags

Nanoparticle Tracking Analysis Extracellular Vesicles NTA Parameters Sample Preparation Cuvette Particle Concentration Dilution Focus Frame Rate Exposure Time Stir Time Reproducibility Standardization

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