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Journal
/
Bioengineering
/
使用微生物滴培养系统(MMC)的自动微生物培养和适应性进化
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Bioengineering
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JoVE Journal
Bioengineering
Automated Microbial Cultivation and Adaptive Evolution using Microbial Microdroplet Culture System (MMC)
Please note that all translations are automatically generated.
Click here for the English version.
使用微生物滴培养系统(MMC)的自动微生物培养和适应性进化
DOI:
10.3791/62800-v
•
08:18 min
•
February 18, 2022
•
Xingjin Jian*
1,2
,
Xiaojie Guo*
1,2,3
,
Jia Wang
,
Zheng Lin Tan
2,6
,
Xin-hui Xing
2,5
,
Liyan Wang
,
Chong Zhang
2,5
1
Department of Chemical Engineering, Institute of Biochemical Engineering
,
Tsinghua University
,
2
Key Laboratory of Industrial Biocatalysis, Ministry of Education
,
Tsinghua University
,
3
Luoyang TMAXTREE Biotechnology Co., Ltd.
,
4
Biochemical Engineering Research Group, School of Chemical Engineering and Technology
,
Xi’an Jiaotong University
,
5
Center for Synthetic & Systems Biology
,
Tsinghua University
,
6
School of Life Science and Technology
,
Tokyo Institute of Technology
Chapters
00:04
Introduction
00:51
Preparations for the Experiment
01:48
Growth Curve Measurement in Microbial Microdroplet Culture System (MMC)
04:25
Adaptive Evolution in Microbial Microdroplet Culture System (MMC)
06:41
Results: The Adaptive Evolution of the Methanol-Essential
Escherichia coli
(MeSV2.2) in MMC
07:36
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议描述了如何使用微生物滴培养系统(MMC)进行自动微生物培养和适应性进化。MMC可以自动连续地培养和分培养微生物,并在线监测其生长,具有相对较高的通量和良好的并行化性,减少了劳动力和试剂消耗。
Tags
Automated Microbial Cultivation
Adaptive Evolution
Microbial Microdroplet Culture System (MMC)
Low-cost
Operation-friendly
Reliable Results
Simple Operations
Microbiology Research
Growth Curve Measurement
Adaptive Laboratory Evolution
Single Factor Multi-level Analysis
Microfluidic Chip
Escherichia Coli MG1655
Cultivation Temperature
Photoelectric Signal
MMC Oil
Initial Bacteria Solution
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