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使用微生物滴培养系统(MMC)的自动微生物培养和适应性进化
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Automated Microbial Cultivation and Adaptive Evolution using Microbial Microdroplet Culture System (MMC)
DOI:

08:18 min

February 18, 2022

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Chapters

  • 00:04Introduction
  • 00:51Preparations for the Experiment
  • 01:48Growth Curve Measurement in Microbial Microdroplet Culture System (MMC)
  • 04:25Adaptive Evolution in Microbial Microdroplet Culture System (MMC)
  • 06:41Results: The Adaptive Evolution of the Methanol-Essential Escherichia coli (MeSV2.2) in MMC
  • 07:36Conclusion

Summary

Automatic Translation

该协议描述了如何使用微生物滴培养系统(MMC)进行自动微生物培养和适应性进化。MMC可以自动连续地培养和分培养微生物,并在线监测其生长,具有相对较高的通量和良好的并行化性,减少了劳动力和试剂消耗。

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