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Reconstitution of Msp1 Extraction Activity with Fully Purified Components

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Reconstitution of Msp1 Extraction Activity with Fully Purified Components

Reconstitution of Msp1 Extraction Activity with Fully Purified Components

DOI:

10.3791/62928-v

•

05:52 min

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August 10, 2021

•

Heidi L. Fresenius, Matthew L. Wohlever

¹Department of Chemistry & Biochemistry,University of Toledo

Chapters

00:05Introduction
00:36Reconstitution of Msp1 and Model TA Protein
02:37Extraction Assay
04:47Results: Determining Extraction Efficiency
05:26Conclusion

Summary

Automatic Translation

Here, we present a detailed protocol for reconstitution of Msp1 extraction activity with fully purified components in defined proteoliposomes.

Tags

Msp1 AAA-ATPase Protein Quality Control Reconstitution Lipid Bilayer TA Proteins Liposomes BioBeads Glutathione Spin Columns Extraction Assay SDS-PAGE

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