Formal Correction: Erratum: Myocardial Infarction by Percutaneous Embolization Coil Deployment in a Swine Model
Posted by JoVE Editors on 5/26/2022. Citeable Link.
An erratum was issued for: Myocardial Infarction by Percutaneous Embolization Coil Deployment in a Swine Model. The Protocol and Discussion sections were updated.
Step 3.5 was updated from:
Clean the right femoral area with surgical soap and antiseptic povidone-iodine solution under sterile conditions
to:
Clean the right femoral area with surgical soap followed by alternating antiseptic povidone-iodine solution and alcohol 3 times under sterile conditions.
Section 9 was updated from:
9. Euthanasia method
- Under previous sedation and anesthesia, as previously described, administer an IV sodium thiopental overdose (200 mg/kg).
- Confirm cardiorespiratory arrest and death by monitoring vital signs (electrocardiogram, blood pressure, capnography).
to:
9. Postoperative pain assessment and monitoring
- During the post-surgical follow-up, monitor the general condition of the animals, including the respiratory rate, food and water intake, activity and interaction with the other individuals, appearance and coloration of the skin, and the evolution of the surgical wound.
- Apply a daily supervision protocol according to the following scoring criteria:
- Weight:
0: Normal
1: <10% weight loss
2: 10-20% weight loss
3:> 20% weight loss
- Body condition:
0: Good: non-prominent vertebrae, pelvic or spinal bones
2: Regular: evidence of spinal segmentation, palpable pelvic bones
3: Emaciation: extremely marked skeleton, little or no meat to cover
- Behavior:
0: Normal: Active and interactive in your environment
1: Slight decline in activity and less interactive
2: Abnormal: pronounced decline in activity, isolated
3: Abnormal: Immobile or hyperactivity, possible self-harm
- Physical appearance:
0: Normal: skin/hair shiny and eyes bright
1: Disappears embalming, skin/hair without shine
2: Poor skin/nasal secretions
3: Poor skin, abnormal or hunched posture
- Behavioral disorders:
0: None
1: Inability to move normally
2: Unable to reach food/drink, isolated from other animals
3: Intention to hide/corner, does not respond to stimuli (dying)
- Clinical signs:
0: None
1: Hypothermia, fever, mild respiratory failure
2: Infection of the surgical wound, moderate respiratory failure with muco-bloody secretions
3: Heart failure, severe respiratory failure (cyanosis, open mouth)
Score:
- 1-5: Supervise the animals once a day.
- 6-12: Provide supportive therapy if necessary.
- Any animal with a score of 3 in any of the above parameters or with a total score >12 will be euthanized.
NOTE: The animals should be monitored daily by the animal care staff and twice a week by the research and veterinary team. - Although no pain and distress are expected from the procedure, if any animal shows signs of pain, give analgesic therapy (tramadol, oral, 2-4 mg/kg, daily). If any animal does not respond to analgesic medication and shows signs of chronic pain (very low probability), euthanize the animal with an anesthetic overdose (sodium thiopental, IV, 200 mg/kg).
- If the surgical wound shows signs of infection (low probability) despite the antibiotic therapy administered, treat the wound daily and initiate a new antibiotic regimen (cefquinome sulphate, IM, 2 mg/kg, daily).
10. Euthanasia method
- Under previous sedation and anesthesia, as previously described, administer an IV sodium thiopental overdose (200 mg/kg).
- Confirm cardiorespiratory arrest and death by monitoring vital signs (electrocardiogram, blood pressure, capnography).