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Biochemistry
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亲和色谱法表达和纯化人基质金属蛋白酶-3
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Biochemistry
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JoVE Journal
Biochemistry
Bacterial Expression and Purification of Human Matrix Metalloproteinase-3 using Affinity Chromatography
Please note that all translations are automatically generated.
Click here for the English version.
亲和色谱法表达和纯化人基质金属蛋白酶-3
DOI:
10.3791/63263-v
•
07:32 min
•
March 30, 2022
•
Alexander J. Bolt*
1
,
Linh D. Do*
1
,
Maryam Raeeszadeh-Sarmazdeh
1
Department of Chemical & Materials Engineering
,
University of Nevada, Reno
Chapters
00:04
Introduction
00:52
MMP Expression
03:46
MMP Purification
04:27
MMP Refolding
05:12
MMP Activation
05:46
Results: Purification and Activation of Hisx6-Pro-MMP-3cd From BL21(DE3) and R2DP Cells
06:38
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
His-tag纯化,透析和活化用于提高细菌中可溶性活性基质金属蛋白酶-3催化结构域蛋白表达的产量。蛋白质级分通过SDS-PAGE凝胶进行分析。
Tags
Bacterial Expression
Recombinant MMP-3
Affinity Chromatography
E. Coli
R2DP Cell Strain
MMP Inhibitors
Protein Purification
Protein Folding
Post-translational Processing
IPTG Induction
Cell Lysis
Inclusion Body Solubilization
Sonication
Centrifugation
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