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Expanding the Toolkit for In Vivo Imaging of Axonal Transport
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Expanding the Toolkit for In Vivo Imaging of Axonal Transport
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Expanding the Toolkit for In Vivo Imaging of Axonal Transport

DOI:
10.3791/63471-v

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09:24 min

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December 23, 2021

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, , ,
1Department of Neuromuscular Diseases, UCL Queen Square Institute of Neurology,University College London, 2UCL Queen Square Motor Neuron Disease Centre,University College London, 3UK Dementia Research Institute,University College London

Chapters

  • 00:05Introduction
  • 00:58Presurgery Preparation and Intramuscular Injections
  • 03:16In Vivo Axonal Transport
  • 04:53In Vivo Axonal Imaging
  • 06:55Results: Assessment of In Vivo Axonal Transport of Signaling Endosomes and Mitochondria
  • 08:46Conclusion

Summary

Automatic Translation

Automatic Translation

Using transgenic fluorescent mice, detailed protocols are described to assess in vivo axonal transport of signaling endosomes and mitochondria within motor and sensory axons of the intact sciatic nerve in live animals.

Tags

In Vivo ImagingAxonal TransportSignaling EndosomesMitochondriaPeripheral NeuronsMotor Disease ModelsAgingGlass MicropipetteIntramuscular InjectionsTibialis Anterior MuscleTetanus NeurotoxinSciatic Nerve

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