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Cytotoxicity Assays with Zebrafish Cell Lines
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Cytotoxicity Assays with Zebrafish Cell Lines

Cytotoxicity Assays with Zebrafish Cell Lines

DOI:
10.3791/64860-v

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08:22 min

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January 06, 2023

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, , , , , , , , ,
1Graduate Program in Genetics, Department of Genetics,Federal University of Paraná (UFPR), 2Safety of Product Department,Grupo Boticário, 3Department of Genetics,Federal University of Paraná (UFPR)

Chapters

  • 00:04Introduction
  • 01:02Plating the Cells in 96-Well Plates
  • 02:01Exposing the Cells to Test Chemicals
  • 03:19Performing the Cytotoxicity Assays
  • 05:51Results: Calculation of Cell Viability for Different Test Chemicals
  • 07:39Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol presents commonly used cytotoxicity assays (Alamar Blue [AB], CFDA-AM, Neutral Red, and MTT assays) adapted for the assessment of cytotoxicity in zebrafish embryo (ZEM2S) and liver (ZFL) cell lines in 96-well plates.

Tags

Cytotoxicity AssayZebrafish Cell LinesZEM2SZFL96-well PlateCell ViabilityAcute ToxicityEcotoxicityAlternative Fish AssaysHepatocytesEmbryonic CellsCell SuspensionMulti-channel PipetteTest ChemicalsTriton X-100Exposure Media

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