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Biology

Published: September 15, 2023 doi: 10.3791/65136
Automatic Translation
1,2, 1, 2,3, 2,4, 1, 1, 2
1Center for Vector-borne Infectious Diseases, Department of Microbiology, Immunology, and Pathology, Colorado State University, 2Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 3Department of Biological Sciences, Vanderbilt University, 4National Center for Microbiology, Instituto de Salud Carlos III

Materials

Name Company Catalog Number Comments
1 oz. translucent plastic souffle cups WebstaurantStore 301100PC
2 oz. translucent polystyrene souffle cups WebstaurantStore 760P200N
3 mL plastic pipettors  Cornin 357524
50 mL conical tubes Any brand
64 oz. white double poly-coated paper food cup WebstaurantStore 999SOUP64WB for mosquito enclosement
65 mm lens  Canon MP-E 65mm f/2.8 1-5x Macro Photo Canon Macro Photo MP-E 65mm, 7D-65mm-1X; zoom=1, 200, 6.3, ISO=100; for photographing wings or egg papers, although other cameras are likely sufficient
Aedes aegypti mosquitoes BEI multiple strains as eggs are available
Artifical membrane feeders https://lillieglassblowers.com/ Meduim membrane feeder, Custom made, 33mm Chemglass also offers, but sizes are wrong for us. Ours are about 3 cm?
ATP MP Biomedical ICN15026605 Any good quality ATP, 10mM filter sterile aliquots at -20
Autoclave for sterilizing water for hatching
Canon EOS 7D camera  Canon 3814B004 for photographing wings or egg papers, although other cameras are likely sufficient
defibrinated sheep blood  Colorado Serum Co. 60 ml, every 2 weeks https://colorado-serum-com.3dcartstores.com/sheep-defibrinated
Dual Gooseneck Microscope Illuminator Dolan Jenner Fiber-Lite 180 181-1 System
Ethanol
Forceps Dumont 5SF
Gauze omnisorb ii 4" non-woven sponges
glass microscope slide Fisher Scientific 12-544-2
Glass Petri dishes, 100 × 15 mm VWR 75845-546 for anesthesizing/manipulating mosquitoes on ice
Hogs' gut Any Deli we buy in bulk, split, wash and store in small aliquots of ~4X12" at -20 in 50 ml conical
Ice
Ice bucket
Kimwipe Fisher Scientific 06-666A
Leica GZ4 StereoZoom microscope for screening transgenic mosquitoes (if applicable)
Paintbrush AIT synthetic brush  size 10-0 for manipulating larvae/pupae (Amazon)
Panty hose Walmart L'eggs Everyday  Women's Nylon Plus Knee Highs Sheer Toe, 16 pairs (plus fits the carton)
Pencils Any brand
Plastic containers for 2° storage of cartons Walmart Sterilite 58 Qt Storage Box Clear Base White Lid Set of 8
Plastic containers for growing larvae Walmart Sterilite 28 Qt. Storage Box Plastic, White, Set of 10
Plastic containers for hatching larvae Walmart Sterilite 6 Qt. Storage Box Plastic, White
polypropylene clear deli containers  WebstaurantStore  127DM12BULK 12 oz, or 16 oz if needed for bigger (127RD16BULK)
Rubber bands Office Max  #100736/#909606 /#3777415 12", #64 and #10
Rubber stopper VWR 217-0515 for mosquito enclosement
Sugar source, such as sugar cubes or raisins
Tetramin flake food Tetramin 16106
tpsDig Stony Brook Morphometrics A free morphometric image-processing software distributed online available at https://www.sbmorphometrics.org/
tpsUtil Stony Brook Morphometrics A free morphometric image-processing software distributed online available at https://www.sbmorphometrics.org/
White organza fabric 8” × 8” FabricWholesale.com  4491676 Joann Casa Collection Organza Fabric by Casa Collection 
Whitman Grade 1 Qualitative Filter paper  Whitman 1001-824 for egg papers. The white color makes it easier to see the black eggs.

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References

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Cite this Article

Williams, A. E., Sanchez-Vargas, I., … More

Williams, A. E., Sanchez-Vargas, I., Martin, L. E., Martin-Martin, I., Bennett, S., Olson, K. E., Calvo, E. Quantifying Fitness Costs in Transgenic Aedes aegypti Mosquitoes. J. Vis. Exp. (199), e65136, doi:10.3791/65136 (2023).

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