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Medicine

Published: October 27, 2023 doi: 10.3791/66016

Materials

Name Company Catalog Number Comments
Animal
C57/B6J mice Gempharmatech Experimental Animals Company C57/B6J For the establishment of pulp exposure
Equipment
1 mL syringe Chengdu Xinjin Shifeng Medical Apparatus & Instruments Co. LTD. SB1-074(IV) Apply in drug injection.
8# C+ file Readysteel 0010047 Apply in exposing the roof of pulp chamber.
Anesthesia Mix solution 10% ketamine hydrochloride+ 5% xylazine + 85% sterile isotonic saline. 
DAPI Staining Solution Beyotime C1005 Apply in immunofluorescence staining for counter-staining of nucleus.
Dental high-speed dental handpiece Jing yuan electronic commerce technology WJ-422 Apply in pulp exposure.
Heavy wire cutter Jirui Medical Instrument Co., Ltd. none Apply inarc cutting.
Hematoxylin and Eosin Stain kit Biosharp BL700B For the histological analysis of the slides.
IL-6 antibody Novus NBP2-89149 Apply in immunofluorescence staining to detect the inflammation of the dental pulp.
Ketamine(Ketamine hydrochloride) Vet One, Boise, Idaho, USA C3N VT1 100mg/kg, IP. Apply in nesthetization.
Medical tap 3M 1530 Apply in mice immobilization.
Orthodontic arch wire  Shanghai Wei Rong Medical Apparatus Co. LTD. K417 Diameter of 8µm
Round dental burr (0.6 mm) Shofu global 072208 Apply in removing enamel and shallow layer of dentin.
Young loop bending plier Jirui Medical Instrument Co., Ltd. none Apply in arc bending.

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References

  1. Kleinert, A., Kleinert, L., Ozimirska, M., Chałas, R. Endodontium - together or separately. Folia Morphol. 77 (3), 409-415 (2018).
  2. Dhillon, H., Kaushik, M., Sharma, R. Regenerative endodontics-Creating new horizons. J Biomed Mater Res B Appl Biomater. 104 (4), 676-685 (2016).
  3. Prati, C., Pirani, C., Zamparini, F., Gatto, M. R., Gandolfi, M. G. A 20-year historical prospective cohort study of root canal treatments. A Multilevel analysis. Int Endod J. 51 (9), 955-968 (2018).
  4. Su, Y., Wang, C., Ye, L. Healing rate and post-obturation pain of single- versus multiple-visit endodontic treatment for infected root canals: a systematic review. J Endod. 37 (2), 125-132 (2011).
  5. Murray, P. E., Garcia-Godoy, F., Hargreaves, K. M. Regenerative endodontics: a review of current status and a call for action. J Endod. 33 (4), 377-390 (2007).
  6. Arora, S., et al. Potential application of immunotherapy for modulation of pulp inflammation: opportunities for vital pulp treatment. Int Endod J. 54 (8), 1263-1274 (2021).
  7. Eramo, S., Natali, A., Pinna, R., Milia, E. Dental pulp regeneration via cell homing. Int Endod J. 51 (4), 405-419 (2018).
  8. Hasan, A., et al. Expression of Toll-like receptor 2, Dectin-1, and Osteopontin in murine model of pulpitis. Clin Oral Investig. 27 (3), 1177-1192 (2023).
  9. Wang, Y., et al. DDIT3 aggravates pulpitis by modulating M1 polarization through EGR1 in macrophages. Int Immunopharmacol. 120, 110328 (2023).
  10. Richert, R., et al. A critical analysis of research methods and experimental models to study pulpitis. Int Endod J. 55 (Suppl 1), 14-36 (2022).
  11. Huang, X. F., Zhao, Y. B., Zhang, F. M., Han, P. Y. Comparative study of gene expression during tooth eruption and orthodontic tooth movement in mice. Oral Dis. 15 (8), 573-579 (2009).
  12. Kwon, S. J., et al. Thermal irritation of teeth during dental treatment procedures. Restor Dent Endod. 38 (3), 105-112 (2013).
  13. He, Y., et al. Pulpal tissue inflammatory reactions after experimental pulpal exposure in mice. J Endod. 43 (1), 90-95 (2017).
  14. Karrar, R. N., et al. Molecular biomarkers for objective assessment of symptomatic pulpitis: A systematic review and meta-analysis. Int Endod J. 56 (10), 1160-1177 (2023).
  15. Goldman, E., Reich, E., Abramovitz, I., Klutstein, M. Inducing apical periodontitis in mice. J Vis Exp. (150), e59521 (2019).
  16. Duncan, H. F. Present status and future directions-Vital pulp treatment and pulp preservation strategies. Int Endod J. 55 (Suppl 3), 497-511 (2022).
  17. Shi, X., Li, Z., He, Y., Jiang, Q., Yang, X. Effect of different dental burs for experimental induction of pulpitis in mice. Arch Oral Biol. 83, 252-257 (2017).
  18. Du, W., et al. Indigenous microbiota protects development of medication-related osteonecrosis induced by periapical disease in mice. Int J Oral Sci. 14 (1), 16 (2022).
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Cite this Article

Tang, Y., Yu, C., Li, F., Wang, H.,More

Tang, Y., Yu, C., Li, F., Wang, H., Ye, L. Establishment of a Murine Pulp Exposure Model with a Novel Mouth-Gag for Pulpitis Research. J. Vis. Exp. (200), e66016, doi:10.3791/66016 (2023).

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