Materials
Name | Company | Catalog Number | Comments |
Animal | |||
C57/B6J mice | Gempharmatech Experimental Animals Company | C57/B6J | For the establishment of pulp exposure |
Equipment | |||
1 mL syringe | Chengdu Xinjin Shifeng Medical Apparatus & Instruments Co. LTD. | SB1-074(IV) | Apply in drug injection. |
8# C+ file | Readysteel | 0010047 | Apply in exposing the roof of pulp chamber. |
Anesthesia Mix solution | 10% ketamine hydrochloride+ 5% xylazine + 85% sterile isotonic saline. | ||
DAPI Staining Solution | Beyotime | C1005 | Apply in immunofluorescence staining for counter-staining of nucleus. |
Dental high-speed dental handpiece | Jing yuan electronic commerce technology | WJ-422 | Apply in pulp exposure. |
Heavy wire cutter | Jirui Medical Instrument Co., Ltd. | none | Apply inarc cutting. |
Hematoxylin and Eosin Stain kit | Biosharp | BL700B | For the histological analysis of the slides. |
IL-6 antibody | Novus | NBP2-89149 | Apply in immunofluorescence staining to detect the inflammation of the dental pulp. |
Ketamine(Ketamine hydrochloride) | Vet One, Boise, Idaho, USA | C3N VT1 | 100mg/kg, IP. Apply in nesthetization. |
Medical tap | 3M | 1530 | Apply in mice immobilization. |
Orthodontic arch wire | Shanghai Wei Rong Medical Apparatus Co. LTD. | K417 | Diameter of 8µm |
Round dental burr (0.6 mm) | Shofu global | 072208 | Apply in removing enamel and shallow layer of dentin. |
Young loop bending plier | Jirui Medical Instrument Co., Ltd. | none | Apply in arc bending. |
References
- Kleinert, A., Kleinert, L., Ozimirska, M., Chałas, R. Endodontium - together or separately. Folia Morphol. 77 (3), 409-415 (2018).
- Dhillon, H., Kaushik, M., Sharma, R.
Regenerative endodontics-Creating new horizons. J Biomed Mater Res B Appl Biomater. 104 (4), 676-685 (2016). - Prati, C., Pirani, C., Zamparini, F., Gatto, M. R., Gandolfi, M. G. A 20-year historical prospective cohort study of root canal treatments. A Multilevel analysis. Int Endod J. 51 (9), 955-968 (2018).
- Su, Y., Wang, C., Ye, L. Healing rate and post-obturation pain of single- versus multiple-visit endodontic treatment for infected root canals: a systematic review. J Endod. 37 (2), 125-132 (2011).
- Murray, P. E., Garcia-Godoy, F., Hargreaves, K. M. Regenerative endodontics: a review of current status and a call for action. J Endod. 33 (4), 377-390 (2007).
- Arora, S., et al. Potential application of immunotherapy for modulation of pulp inflammation: opportunities for vital pulp treatment. Int Endod J. 54 (8), 1263-1274 (2021).
- Eramo, S., Natali, A., Pinna, R., Milia, E. Dental pulp regeneration via cell homing. Int Endod J. 51 (4), 405-419 (2018).
- Hasan, A., et al. Expression of Toll-like receptor 2, Dectin-1, and Osteopontin in murine model of pulpitis. Clin Oral Investig. 27 (3), 1177-1192 (2023).
- Wang, Y., et al. DDIT3 aggravates pulpitis by modulating M1 polarization through EGR1 in macrophages. Int Immunopharmacol. 120, 110328 (2023).
- Richert, R., et al. A critical analysis of research methods and experimental models to study pulpitis. Int Endod J. 55 (Suppl 1), 14-36 (2022).
- Huang, X. F., Zhao, Y. B., Zhang, F. M., Han, P. Y. Comparative study of gene expression during tooth eruption and orthodontic tooth movement in mice. Oral Dis. 15 (8), 573-579 (2009).
- Kwon, S. J., et al. Thermal irritation of teeth during dental treatment procedures. Restor Dent Endod. 38 (3), 105-112 (2013).
- He, Y., et al. Pulpal tissue inflammatory reactions after experimental pulpal exposure in mice. J Endod. 43 (1), 90-95 (2017).
- Karrar, R. N., et al. Molecular biomarkers for objective assessment of symptomatic pulpitis: A systematic review and meta-analysis. Int Endod J. 56 (10), 1160-1177 (2023).
- Goldman, E., Reich, E., Abramovitz, I., Klutstein, M.
Inducing apical periodontitis in mice. J Vis Exp. (150), e59521 (2019). - Duncan, H. F. Present status and future directions-Vital pulp treatment and pulp preservation strategies. Int Endod J. 55 (Suppl 3), 497-511 (2022).
- Shi, X., Li, Z., He, Y., Jiang, Q., Yang, X. Effect of different dental burs for experimental induction of pulpitis in mice. Arch Oral Biol. 83, 252-257 (2017).
- Du, W., et al. Indigenous microbiota protects development of medication-related osteonecrosis induced by periapical disease in mice. Int J Oral Sci. 14 (1), 16 (2022).