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Chapters
- 00:17Introduction
- 01:10Dissection of the Bulge from Adult Mouse Whisker Follicles
- 04:34Culture of Bulge Explants
- 07:18Conclusion
Aqui nós mostramos o nosso método para isolar do mouse epidérmicas células da crista neural haste (EPI-NCSC). Técnica envolve a micro-dissecando folículos bigode, isolando o bojo e placeing-lo em cultura de tecidos. EPI-NCSC começam a emigrar a partir de explantes saliência para o substrato dentro de 3-4 dias.
Tags
Mouse Epidermal Neural Crest Stem CellEPI-NCSCEmbryonic Neural CrestHair FolliclesMultipotent Stem CellsDifferentiated Cell TypesNeuronsNerve Supporting CellsSmooth Muscle CellsBone/cartilage CellsMelanocytesHairy SkinPure Population Of Stem CellsProtocolWhisker FolliclesWhisker PadCollagen CapsuleCulture PlateEmigration
