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Detection and Isolation of Viable Mouse IL-17-Secreting T Cells

JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology

Detection and Isolation of Viable Mouse IL-17-Secreting T Cells

Detection and Isolation of Viable Mouse IL-17-Secreting T Cells

DOI:

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12:38 min

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December 18, 2008

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Anna Foerster, Mario Assenmacher, Michaela Niemoeller, Elly Rankin, Mariette Mohaupt, Anne Richter

¹Miltenyi Biotec,GmbH

Chapters

00:00Title
01:30Introduction
02:10Preparing Reagents
03:07Stimulating the Splenocytes
05:19Labelling Cells with the Catch Reagent
07:36Labeling Cells with IL-17 Detection Antibody (Biotin) and Anti-Biotin-PE
09:50FACS Analysis
11:40Conclusion

Summary

Automatic Translation

This procedure describes the detection and isolation of mouse TH17 leukocytes that actively secrete IL-17 upon stimulation.

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