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Poziome Przygotowanie Kromka Retina
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JoVE Journal Biology
Horizontal Slice Preparation of the Retina

Poziome Przygotowanie Kromka Retina

DOI:
10.3791/108-v

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10:45 min

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November 20, 2006

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, ,
1Dpt of Physiology and Biophysics,Dalhousie University, 2Massachusetts General Hospital,Harvard Medical School

Chapters

  • 00:05Tools
  • 00:22Preparation of agar block and agarose gel
  • 00:51Isolation of retina
  • 02:06Preparation of retina slices
  • 10:17Examples: cell layer of retina

Summary

Automatic Translation

Automatic Translation

Tradycyjnie w pionie kawałek i cały montażu przygotowanie siatkówce były używane w badaniach funkcji siatkówki obwodów. Poniżej opiszemy powieści krojenia metody zachowania dendrytycznych morfologii neuronów siatkówki nienaruszone.

Tags

Horizontal SliceRetinaRetinal CircuitsAmacrine CellsDendritesVertical SliceWhole-mount PreparationMorphologyPatch-clamp RecordingsGlial CellsMueller CellsSlicing MethodVibratome SlicerLow-temperature Melting Agarose GelFunction Of Retinal NeuronsCalcium Imaging TechniqueImmunocytochemistrySingle-cell RT-PCR

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