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Rening och visualisering av influensa A Viral Ribonucleoprotein Complexes
JoVE Journal
Biology
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JoVE Journal Biology
Purification and Visualization of Influenza A Viral Ribonucleoprotein Complexes
DOI:

09:35 min

February 09, 2009

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Chapters

  • 00:00Title
  • 00:58Introduction
  • 01:27Disruption of the Influenza A Virion
  • 02:53Glycerol Gradient Sediment Velocity Centrifugation
  • 04:18Analysis of the Glycerol Gradient Fractions by SDS Polyacrylamide Gel Electrophoresis
  • 05:40Concentration of the vRNP Fractions
  • 06:39Negative Staining of vRNPs
  • 08:25Representative Results/ Outcome
  • 09:05Conclusion

Summary

Automatic Translation

Genomet av influensa A-virus består av åtta separata komplex av RNA och proteiner, kallade virala ribonucleoprotein komplex (vRNPs). Detta dokument beskriver rening glycerol lutning och transmissionselektronmikroskopi visualisering av influensa A vRNPs.

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