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Measuring Near Plasma Membrane and Global Intracellular Calcium Dynamics in Astrocytes
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Measuring Near Plasma Membrane and Global Intracellular Calcium Dynamics in Astrocytes

Measuring Near Plasma Membrane and Global Intracellular Calcium Dynamics in Astrocytes

DOI:
10.3791/1142-v

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12:48 min

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April 26, 2009

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1Departments of Physiology and Neurobiology,David Geffen School of Medicine, University of California, Los Angeles

Chapters

  • 00:00Title
  • 00:58Introduction
  • 01:56Preparing Hippocampal Astrocyte Culture
  • 06:33Calcium Imaging
  • 09:18Results
  • 11:26Conclusion

Summary

Automatic Translation

Automatic Translation

We describe how to measure near membrane and global intracellular calcium dynamics in cultured astrocytes using total internal reflection and epifluorescence microscopy.

Tags

Near Plasma MembraneGlobal Intracellular Calcium DynamicsAstrocytesGlial CellsBrain FunctionFunctional InteractionsSecond MessengerTransmittersCalcium ElevationsTotal Internal Reflection Fluorescence MicroscopyTIRF MicroscopyLive CellsAstrocyte Calcium Signaling

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