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Chapters
- 00:00Title
- 00:56Introduction
- 01:25‘Inside-Out’ Tissue Preparation
- 04:29Neuronal Bouton Labeling with Fluorescently Labeled Primary Antibody Against HRP
- 05:23Perisynaptic Space Labeling with Dextran Fluorophore Conjugate
- 06:12Mounting the Tissue for Visualization Under a Confocal Microscope
- 07:23Representative Results
- 10:17Conclusion
हम एक उपन्यास अंदर बाहर रहते मक्खी लार्वा confocal माइक्रोस्कोपी के साथ फ्लोरोसेंट रंजक के उपयोग के ऊतक की तैयारी में Glia-neuromuscular synapses के संरचनात्मक सुविधाओं का वर्णन किया. हम लेबल एचआरपी फ्लोरोसेंट प्राथमिक एंटीबॉडी के साथ रहते न्यूरॉन टर्मिनलों, और भी फ्लोरोसेंट Dextrans साथ perisynaptic अंतरिक्ष visualized.
Tags
VisualizingLiveDrosophilaGlial-neuromuscular JunctionFluorescent DyesGFP Labeled Glial StructuresDeveloping Larval Fly Neuromuscular SynapseLive Glial-nerve-muscle SynapsesOptical PropertiesAccess Of PerfusatesFly LarvaeArtificial HemolymphRhythmic Body ContractionsDissectionPosterior SegmentsBlunt Insect PinEverted Larval Body WallUltra-fine Dissection ScissorsVisceral Side Of Body Wall MusclesGlial Structures At NMJMembrane Targeted GFPGlial Specific PromotersPost-synaptic MembraneSSR (Subsynaptic Reticula)Muscle Expressed Synaptically Targeted DsRedAcutely Label Motor Neuron TerminalsPrimary Antibodies To HRPDye Diffusion PropertiesPerisynaptic Space
