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Biology
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住在果蝇眼睛成虫光盘胶质细胞迁移成像
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Biology
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JoVE Journal
Biology
Live Imaging of Glial Cell Migration in the Drosophila Eye Imaginal Disc
Please note that all translations are automatically generated.
Click here for the English version.
住在果蝇眼睛成虫光盘胶质细胞迁移成像
DOI:
10.3791/1155-v
•
09:12 min
•
July 09, 2009
•
Patrick Cafferty
,
Xiaojun Xie
,
Kristen Browne
,
Vanessa J. Auld
1
Department of Zoology
,
University of British Columbia – UBC
Chapters
00:00
Title
00:57
Introduction
01:28
Pre-experiment Set Up
03:13
Dissection of the Drosophila Eye-Brain Complex
06:22
Mounting the Eye Imaginal Disc in a Magnetic Culture Chamber
07:12
Visualization of Migrating Glial Cells in the Eye Imaginal Disc
07:41
Representative Results: Time-lapse Confocal Images
08:53
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们描述了一个协议,以研究胶质细胞迁移到发展果蝇眼睛使用现场的微观分析与GFP标记的神经胶质细胞配对。
Tags
Live Imaging
Glial Cell Migration
Drosophila Eye Imaginal Disc
Vertebrate Organisms
Invertebrate Organisms
Final Target Regions
Ensheath And Support Neurons
Live Migration
Developing Pupal Wing
Microscopic Analysis
Motile Cells
Cellular Behavior
Rate Of Growth
Changes In Direction
Genetic Tools
Specific Genes
Development Process
Optic Stalk
Eye Imaginal Disc
GFP Expression
Wild Type
Mutant Animals
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