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Chapters
- 00:05Title
- 01:07Introduction
- 01:46In utero Retinal Electroporation – Injecting and Electroporating DNA into E14.5 Murine Retinae in vivo
- 08:40In utero Retinal Electroporation – Retrieval of Electroporated Retina at E18.5
- 12:34Ex vivo Retinal Electroporation – Injecting and Electroporating DNA into E14.5 Murine Retinae in vitro
- 18:49Ex vivo Retinal Electroporation – Harvesting and Plating of GFP+ Retinal Explants (2 days post-electroporation)
- 23:33Representative Results from in utero and ex vivo Retinal Electroporations
- 25:23Conclusion
Nous présentons ici deux techniques pour la manipulation de l'expression génique dans des cellules murines ganglionnaires de la rétine (CGR) en<em> In utero</em> Et<em> Ex vivo</em> Électroporation. Ces techniques permettent d'examiner comment des altérations de l'expression des gènes affectent le développement du CJR, guidage axonal, et les propriétés fonctionnelles.
Tags
In Utero ElectroporationEx Vivo ElectroporationGene ExpressionMouse Retinal Ganglion CellsRetinal DevelopmentAxon GuidanceRetinotopic OrganizationSynapse FormationTransgenic MiceGene ManipulationChick In Ovo ElectroporationNeonatal Mouse Pups ElectroporationAdult Rats ElectroporationEmbryonic Murine Retinae ElectroporationRGC DevelopmentAxon ProjectionsIn Vivo Electroporation
