Journal
/
Biology
/
Harvesting and Preparing Drosophila Embryos for Electrophysiological Recording and Other Procedures
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Harvesting and Preparing Drosophila Embryos for Electrophysiological Recording and Other Procedures

Harvesting and Preparing Drosophila Embryos for Electrophysiological Recording and Other Procedures

DOI:
10.3791/1347-v

•

08:22 min

•

May 20, 2009

•

, ,
1Department of Biological Sciences,University of Illinois, 2Department of Biological Sciences,Vanderbilt University

Chapters

  • 00:04Title
  • 00:41Introduction
  • 01:21Embryo Collection
  • 04:40Dissection Preparation
  • 07:36Conclusion

Summary

Automatic Translation

Automatic Translation

This technique exposes the Drosophila embryonic neuromusculature for immunohistochemistry or electrophysiological recording. It is useful for studying early events in neuromuscular development or performing electrophysiology in mutants that cannot hatch.

Tags

DrosophilaEmbryosElectrophysiological RecordingProceduresGenetic ModelEmbryonic DevelopmentFunctional NeuroscienceDevelopmental ProgramsElectrical Signaling PropertiesChemical SynapsesNeural Circuit FormationInvestigationLate Developmental PeriodEpidermal CuticleSurgical GlueControlled DissectionDorsal Longitudinal IncisionVentral Nerve CordBody Wall MusculatureGenetic MutantsSynapse FormationMolecular Mechanisms

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/1347?article_type=v

Harvesting Sperm and Artificial Insemination of Mice
check_url/1347?article_type=v

Preparation of Neuronal Cultures from Midgastrula Stage Drosophila Embryos
check_url/1347?article_type=v

Electrophysiological Methods for Recording Synaptic Potentials from the NMJ of Drosophila Larvae
check_url/1347?article_type=v

Electrophysiological Recording in the Drosophila Embryo
check_url/1347?article_type=v

Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube
check_url/1347?article_type=v

In-vivo Centrifugation of Drosophila Embryos
check_url/1347?article_type=v

Upright Imaging of Drosophila Embryos
check_url/1347?article_type=v

Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells
check_url/1347?article_type=v

Isolering og rensing av kinesin fra Drosophila Embryoer
check_url/1347?article_type=v

Forbereder Individuell Drosophila Egg Chambers for Live Imaging

Read Article