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Chapters
- 00:05Title
- 01:08Introduction
- 01:40Preparing a Master Mold of the Neuron Culture Device
- 05:16Neuron Culture Device Replication
- 07:11Neuron Culture Device Assembly
- 09:02Cell Loading and Neuron-gila Co-culture
- 12:07Neuron-gila Co-culture in a Microfluidic Device Representative Results
- 12:48Conclusion
We developed a novel multi-compartment neuron co-culture microsystem platform for in vitro CNS axon-glia interaction research. The platform is capable of conducting up to six independent experiments in parallel and was fabricated using a newly developed macro/micro hybrid fabrication method.
Tags
Multi-compartmentCNS Neuron-glia Co-cultureMicrofluidic PlatformIn Vitro ResearchAxon-glia InteractionHigher-throughputFabrication MethodMicro And Macro Scale StructuresSoft-lithography ProcessMass FabricationRepeatabilitySoma CompartmentAxon/glia CompartmentsOligodendrocytes (OLs)Axon-guiding MicrochannelsPhysical BarriersLocalized Axon-glia Interaction StudiesFluidic IsolationPoly(dimethylsiloxane) (PDMS)Biomedical MicrodevicesReservoirs
