A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:08Introduction
- 01:40Preparing a Master Mold of the Neuron Culture Device
- 05:16Neuron Culture Device Replication
- 07:11Neuron Culture Device Assembly
- 09:02Cell Loading and Neuron-gila Co-culture
- 12:07Neuron-gila Co-culture in a Microfluidic Device Representative Results
- 12:48Conclusion
Vi utvecklade en roman med flera fack neuron co-kulturen mikrosystem plattform för in vitro CNS axon-Glia samspelet forskning. Plattformen är kapabel att genomföra upp till sex oberoende experiment parallellt och var fabricerade med hjälp av en nyutvecklad makro / mikro-hybrid tillverkning metod.
Tags
Multi-compartmentCNS Neuron-glia Co-cultureMicrofluidic PlatformIn Vitro ResearchAxon-glia InteractionHigher-throughputFabrication MethodMicro And Macro Scale StructuresSoft-lithography ProcessMass FabricationRepeatabilitySoma CompartmentAxon/glia CompartmentsOligodendrocytes (OLs)Axon-guiding MicrochannelsPhysical BarriersLocalized Axon-glia Interaction StudiesFluidic IsolationPoly(dimethylsiloxane) (PDMS)Biomedical MicrodevicesReservoirs
