Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Preparation and Culture of Rat Lens Epithelial…
JoVE Journal
Biology

This content is Open Access.

JoVE Journal Biology
Preparation and Culture of Rat Lens Epithelial Explants for Studying Terminal Differentiation
 
Click here for the English version

Preparation and Culture of Rat Lens Epithelial Explants for Studying Terminal Differentiation

Article DOI: 10.3791/1519-v • 15:03 min • September 22nd, 2009
September 22nd, 2009 •
1, 1, 1
1Laboratory of Molecular and Developmental Biology, National Eye Institute (NEI), National Institutes of Health (NIH)

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Explants of the central region of rat lens epithelia differentiate synchronously when cultured in the presence of FGF-2. Immunofluorescence microscopy of such cultures can provides novel information about gene expression and signaling events associated with terminal differentiation.

Tags

Preparation Culture Rat Lens Epithelial Explants Terminal Differentiation Anterior Surface Ocular Lens Epithelial Cells Proliferation Annular Zone Ciliary Body Migration Posterior Array Lens Fiber Cells Bulk Of The Lens In Vitro Induction FGF-2 Neonatal Rats Lens Capsule Dissecting Tweezers Tissue Culture Dish Peripheral Regions Central Area FGF-2 Concentration Time Course Signaling Gene Expression Molecular Techniques Biochemical Techniques Pharmacological Techniques Immunofluorescence Microscopy
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter