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Methods for the Study of the Zebrafish Maxillary Barbel
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Methods for the Study of the Zebrafish Maxillary Barbel

Methods for the Study of the Zebrafish Maxillary Barbel

DOI:
10.3791/1558-v

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09:30 min

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November 23, 2009

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,
1Department of Biological Sciences,DePaul University, 2Children’s Memorial Research Center, Department of Pediatrics,Northwestern University Feinberg School of Medicine

Chapters

  • 00:00Title
  • 00:42Introduction
  • 01:07Barbel Development
  • 01:47Barbel Clipping
  • 04:38Agar Embedding of Matched Barbel Pairs
  • 09:08Conclusion

Summary

Automatic Translation

Automatic Translation

The zebrafish maxillary barbel is an integumentary sense organ containing ectodermal, mesodermal and neural crest derivatives. Importantly, the adult barbel can regenerate after proximal amputation. This video introduces maxillary barbel development and demonstrates a surgical protocol to induce regeneration, followed by collection, embedding and downstream imaging of barbel specimens.

Tags

ZebrafishMaxillary BarbelStudy MethodsSkin Sensory AppendagesEctodermal CellsMesodermal CellsNeural Crest CellsVisualizationDevelopmentMaintenanceLife CycleSurgical ProtocolRegeneration InductionAmputation PlaneRegenerative GrowthBarbel AnalysisDNA Electrophoresis

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