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Chapters
- 00:00Title
- 00:56Introduction
- 01:20Removing the Aorta
- 03:43Preparing the Slices or “Rings”
- 06:24Setting Up the Assay and Evaluating the Results
- 07:57Aortic Ring Assay Angiogenesis Representative Results
- 08:50Conclusion
Angiogenesis, the sprouting of blood vessels from pre-existing vasculature, is associated with both natural and pathological processes. Here we demonstrate an aortic ring assay that allows angiogenic potentiators and inhibitors to be directly added to aortic rings in culture. Sprouting and neovessel outgrowth can be determined by inspecting the aortic rings over a period of 6-12 days.
Tags
Aortic Ring AssayAngiogenesisBlood VesselsEndothelial CellsOrgan CultureAortaBasement Matrix Extract (BME)Angiogenic FactorsInhibitors Of AngiogenesisCo-cultureParacrine Angiogenic EffectsSproutingStereomicroscopeNeovessel OutgrowthPhase MicroscopySupernatantsAngiogenic MarkersAnti-angiogenic FactorsCell Death MarkersNitrite
