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Extraction of High Molecular Weight Genomic DNA from Soils and Sediments

JoVE Journal
Biology

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JoVE Journal Biology

Extraction of High Molecular Weight Genomic DNA from Soils and Sediments

Extraction of High Molecular Weight Genomic DNA from Soils and Sediments

DOI:

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11:24 min

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November 10, 2009

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Sangwon Lee, Steven J. Hallam

¹Department of Microbiology and Immunology,University of British Columbia – UBC

Chapters

00:00Title
00:08Introduction
00:53Cell Lysis
02:21DNA Extraction
08:51Cesium Chloride Gradient Ultracentrifugation
09:46Representative Results/ Outcome
10:32Conclusion

Summary

Automatic Translation

A methodology to isolate high molecular weight and high quality genomic DNA from soil microbial community is described.

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