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Chapters
- 00:00Introduction
- 00:20Isolating bone marrow cells
- 00:21Dissecting mouse to isolate bones
- 01:03Isolating tibia
- 01:40Isolating femur
- 02:12Isolating hip bone
- 02:50Isolating spine
- 04:07Cleaning bones
- 06:37Crushing bones
- 09:33Lineage depletion of bone marrow cells
- 14:06Antibody staining for FACS
- 16:06Transplantation into irradiated mouse
- 18:50Discussion
