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Chapters
- 00:00Title
- 00:55Introduction
- 01:39Preparation for Injection and Fabrication of Injection Pipettes
- 04:54Intranuclear Microinjection
- 12:00Whole-cell Recordings of Currents through Heterologously-Expressed G-protein Coupled Inwardly Rectifying K+ (GIRK) Channels from SCG Neurons
- 12:56Conclusion
Tags
Intranuclear MicroinjectionDNADissociated Adult Mammalian NeuronsProtein FunctionPrimary Neuronal Cell CulturesHeterologous Protein ExpressionElectroporationChemically-mediated TransfectionCDNA ConstructsAdult Sympathetic NeuronsInverted MicroscopeGlass Injection PipetN2(g) Pressure Delivery SystemMicromanipulatorTransfection MethodsSingle Cell StudiesElectrophysiology
