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Chapters
- 00:00Title
- 01:22Introduction
- 01:41Selection of Bait and Appropriate MYTH System
- 03:04Generation of tMYTH Baits by Gap Repair
- 03:07a. Vector Preparation and Amplification of Gene of Interest
- 05:30b. Yeast Transformation and Recovery of Bait Plasmid DNA
- 07:03Bait Validation
- 08:10MYTH Screening
- 08:12a. Large Scale Yeast Transformation
- 11:31b. Bait Dependency Test
- 12:54Representative Results/ Outcome
- 13:12Conclusion
