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Preparation of Drosophila Polytene Chromosome Squashes for Antibody Labeling
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Preparation of Drosophila Polytene Chromosome Squashes for Antibody Labeling

Preparation of Drosophila Polytene Chromosome Squashes for Antibody Labeling

DOI:
10.3791/1748-v

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06:37 min

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February 09, 2010

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, , , ,
1Department of Biochemistry, Biophysics, and Molecular Biology,Iowa State University

Chapters

  • 00:00Title
  • 00:28Introduction
  • 01:05Culture of Third Instar Drosophila Larvae
  • 02:04Squash Preparation of Polytene Chromosomes
  • 05:06Representative Results
  • 06:13Conclusion

Summary

Automatic Translation

Automatic Translation

This video protocol illustrates the squash technique used in the Johansen laboratory to prepare Drosophila polytene chromosomes for antibody labeling.

Tags

DrosophilaPolytene ChromosomesAntibody LabelingChromatin StructureGene RegulationCytological AdvantagesGiant Salivary GlandThird Instar LarvaeReplicative CycleChromatin MorphologyEpigenetic ModificationsTranscription ProcessLabeling Technique

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