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的RNA分离方法的改进火炬松( P。火炬松 L)和其他针叶树种
JoVE Journal
Biology
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JoVE Journal Biology
An Improved Method of RNA Isolation from Loblolly Pine (P. taeda L.) and Other Conifer Species
DOI:

08:25 min

February 22, 2010

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Chapters

  • 00:00Title
  • 00:23Tissue Harvest
  • 00:45Collection of Phloem
  • 01:07Collection of Secondary Xylem
  • 01:26Collection of Reaction Wood: Compression and Opposite Secondary Xylem
  • 02:21Liquid Nitrogen Storage
  • 02:46RNA Isolation: Tissue Preparation
  • 03:20RNA Isolation Day 1
  • 04:41RNA Isolation Day 2
  • 06:35RNA Precipitation
  • 07:16Final Resuspension and Quantification
  • 08:02Credits

Summary

Automatic Translation

许多植物组织中,包括从火炬松的韧皮部及木质部(<em>火炬松</em> L),含有酚和干扰RNA纯化的多糖高的水平。此演示文稿讨论的收获种植现场组织和隔离的芯片和其他基因组分析的RNA质量足够技术。

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