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Journal
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Neuroscience
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胚の脊髄から交連ニューロンの解剖と文化
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord
Please note that all translations are automatically generated.
Click here for the English version.
胚の脊髄から交連ニューロンの解剖と文化
DOI:
10.3791/1773-v
•
12:23 min
•
May 25, 2010
•
Sébastien D. Langlois*
1,2
,
Steves Morin*
1
,
Patricia T. Yam
3,4
,
Frédéric Charron
2,5,6,7
1
Molecular Biology of Neural Development
,
Institut de Recherches Cliniques de Montréal
,
2
Division of Experimental Medicine and Program in Neuroengineering
,
McGill University
,
3
Program in Neuroengineering
,
McGill University
,
4
Montreal Neurological Institute
,
5
Department of Anatomy and Cell Biology
,
McGill University
,
6
Department of Biology
,
McGill University
,
7
Department of Medicine
,
Universite de Montreal – University of Montreal
Chapters
00:00
Title
00:45
Introduction
01:17
Spinal Cord Dissection
05:41
Dorsal Spinal Cord Dissection
06:53
Commissural Neuron Culture
11:28
Representative Cultures And Staining Of Dorsal Spinal Cord Neurons
11:55
Conclusion
Summary
Automatic Translation
English (Original)
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français (French)
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Automatic Translation
このビデオでは、E13ラット背側脊髄から交連神経細胞を細かく分析し、文化に方法を示しています。解離交連ニューロンの軸索成長とガイダンスの細胞および分子メカニズムを研究するのに便利です。
Tags
Dissection
Culture
Commissural Neurons
Embryonic Spinal Cord
Axon Guidance
Dorsal Spinal Cord
Stereotyped Trajectories
Guidance Cues
Turning Assays
Immunochemistry
Biochemistry
E13 Rat
Dissociation
Cell Suspension
Poly-L-lysine-coated Glass Coverslips
In Vitro Studies
Cellular Mechanisms
Molecular Mechanisms
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