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分离和富集大鼠间质干细胞（MSCS）及衍生的干细胞分离单细胞集落
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JoVE Journal Biology
Isolation and Enrichment of Rat Mesenchymal Stem Cells (MSCs) and Separation of Single-colony Derived MSCs
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分离和富集大鼠间质干细胞（MSCS）及衍生的干细胞分离单细胞集落

DOI:
10.3791/1852-v

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08:18 min

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March 22, 2010

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,
1Department of Chemical Engineering and Materials Science,City of Hope Cancer Center

Chapters

  • 00:00Title
  • 00:47Introduction
  • 01:18Isolation of Rat MSCs
  • 02:40Enrichment of Rat MSCs
  • 05:17Separation of Single-colony Derived MSCs
  • 06:36Results of Isolation and Enrichment of Rat MSCs
  • 07:41Conclusion

Summary

Automatic Translation

Automatic Translation

大鼠MSCs从股骨和胫骨分离，然后通过磁性细胞分选富集。排序细胞被证实为流式细胞仪检测表面标志物表达。这些细胞克隆密度培养，形成单菌落，然后这些菌落克隆气瓶分开。

Tags

IsolationEnrichmentRat Mesenchymal Stem CellsMSCsSingle-colony Derived MSCsBone MarrowHematopoietic Stem CellsPlastic AdherenceProtocolRat FemursTibiasMagnetic Cell SortingSurface MarkersCD54CD90Flow Cytometry AnalysisHSC Marker CD45HeterogeneitySubpopulationsCloning CylindersCulture Evaluation

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