Journal
/
/
Cell Electrofusion Visualized with Fluorescence Microscopy
JoVE Journal
Biology
Author Produced
This content is Free Access.
JoVE Journal Biology
Cell Electrofusion Visualized with Fluorescence Microscopy
DOI:

05:02 min

July 01, 2010

, ,

Chapters

  • 00:00Title
  • 00:20Loading the Cells with Cell Trackers CMFDA and CMRA
  • 02:31Electrofusion
  • 03:39Image Acquistion and Determination of the Fusion Yield

Summary

Automatic Translation

In this video we demonstrate efficient electrofusion of cells in vitro by means of modified adherence method using electroporation and the subsequent detection of fused cells visualization with fluorescence microscopy.

Related Videos

Read Article