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Journal
/
Biology
/
3D Culture of Organoids from Murine Intestinal Crypts and Single Stem Cell
/
Kryptisolering fra murin tyndtarm
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Crypt Isolation from Murine Small Intestine
Kryptisolering fra murin tyndtarm
3D Culture of Organoids from Murine Intestinal Crypts and Single Stem Cell
DOI:
10.3791/200007-v
•
05:06 min
•
June 02, 2023
•
Yuta Takase
,
Kazuto Fujishima
3
,
Toshio Takahashi
1
Suntory Foundation for Life Sciences
,
Bioorganic Research Institute
,
2
Institute for Integrated Cell-Material Sciences (KUIAS-iCeMS)
,
Kyoto University
,
3
Faculty of Medicine
,
Osaka Medical and Pharmaceutical University
Tags
Keywords: Crypt Isolation
Murine Small Intestine
PBS Antibiotics
EDTA
Extracellular Matrix
ECM
DMEM
Sorbitol
Cell Strainer
Cell Culture
Centrifugation
Microscopy
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