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Journal
/
Cancer Research
/
Transmitochondrial Cybrid Generation From Suspension-Growing Cancer Cells
/
Isolement mitochondrial pour la génération transmitochondriale de Cybrides
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Mitochondrial Isolation for Transmitochondrial Cybrid Generation
Please note that all translations are automatically generated.
Click here for the English version.
Isolement mitochondrial pour la génération transmitochondriale de Cybrides
Transmitochondrial Cybrid Generation From Suspension-Growing Cancer Cells
DOI:
10.3791/200013-v
•
02:46 min
•
March 17, 2023
•
Ruth Soler-Agesta
,
Joaquín Marco-Brualla
,
Patricio Fernández-Silva
2
,
Pilar Mozas
3
,
Alberto Anel
,
Raquel Moreno Loshuertos
2
1
Department of Biochemistry
,
University of Zaragoza
,
2
Institute for Biocomputation and Physics of Complex Systems
,
University of Zaragoza
,
3
Sequencing and Functional Genomics Unit
,
University of Zaragoza-IACS
Tags
Mitochondrial Isolation
Transmitochondrial Cybrid Generation
Centrifugation
Homogenization
Hypotonic Buffer
Mitochondrial Protein Quantification
Bradford Assay
Nuclear Contamination Assessment
Quantitative PCR
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