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Mitochondriale Isolierung für die transmitochondriale Cybrid-Generierung
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Cancer Research
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JoVE Journal
Cancer Research
Mitochondrial Isolation for Transmitochondrial Cybrid Generation
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Mitochondriale Isolierung für die transmitochondriale Cybrid-Generierung
DOI:
10.3791/200013-v
•
02:46 min
•
March 17, 2023
•
Ruth Soler-Agesta
,
Joaquín Marco-Brualla
,
Patricio Fernández-Silva
2
,
Pilar Mozas
3
,
Alberto Anel
,
Raquel Moreno Loshuertos
2
1
Department of Biochemistry
,
University of Zaragoza
,
2
Institute for Biocomputation and Physics of Complex Systems
,
University of Zaragoza
,
3
Sequencing and Functional Genomics Unit
,
University of Zaragoza-IACS
Tags
Mitochondrial Isolation
Transmitochondrial Cybrid Generation
Centrifugation
Homogenization
Hypotonic Buffer
Mitochondrial Protein Quantification
Bradford Assay
Nuclear Contamination Assessment
Quantitative PCR
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