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Biology
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Direct Synthesis of Gold Nanoparticles for Protein Localization Analysis
/
사파이어 디스크-캐리어 어셈블리의 고압 동결(HPF), 동결 대체 고정(FSF) 및 재수화
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JoVE Journal
Biology
High-Pressure Freezing (HPF), Freeze-Substitution Fixation (FSF) and Rehydration of Sapphire Disc-Carrier Assembly
Please note that all translations are automatically generated.
Click here for the English version.
사파이어 디스크-캐리어 어셈블리의 고압 동결(HPF), 동결 대체 고정(FSF) 및 재수화
Direct Synthesis of Gold Nanoparticles for Protein Localization Analysis
DOI:
10.3791/200046-v
•
03:20 min
•
April 28, 2023
•
Zhaodi Jiang
2
1
National Institute of Biological Sciences, Beijing
,
2
Tsinghua Institute of Multidisciplinary Biomedical Research
,
Tsinghua University
Tags
High-pressure Freezing
HPF
Freeze-substitution Fixation
FSF
Sapphire Disc-carrier Assembly
1-hexadecene
Freeze-substitution Solution
Acetone
Rehydration
Centrifuge Tube
Tweezers
Liquid Nitrogen
Temperature Control
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