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Journal
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Biology
/
Functional Site-Directed Fluorometry in Native Skeletal Muscle Cells
/
Murin İzole İskelet Kas Liflerinde Sistein Mühendisliği Voltaj Kapılı Ca
2 +
Kanallarını Transfekte Etmek için Yapı Tasarımı
JoVE Journal
Biology
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JoVE Journal
Biology
Construct Design for Transfecting Cysteine-Engineered Voltage-Gated Ca
2+
Channels in Murine Isolated Skeletal Muscle Fibers
Please note that all translations are automatically generated.
Click here for the English version.
Murin İzole İskelet Kas Liflerinde Sistein Mühendisliği Voltaj Kapılı Ca
2 +
Kanallarını Transfekte Etmek için Yapı Tasarımı
Functional Site-Directed Fluorometry in Native Skeletal Muscle Cells
DOI:
10.3791/200065-v
•
01:22 min
•
June 02, 2023
•
Hugo Bibollet
,
Daniel F. Bennett
,
Martin F. Schneider
,
Erick O. Hernández-Ochoa
1
Department of Biochemistry and Molecular Biology
,
University of Maryland School of Medicine
Tags
Keywords: Cysteine-engineered Voltage-gated Ca2+ Channels
Murine Isolated Skeletal Muscle Fibers
CaV1.1 CDNA Construct
EGFP-tagged Alpha Subunit
Cytomegalovirus Promoter
Site-directed Mutagenesis
S4 Membrane Extracellular Interface
GFP Observation
Ionic Current Recording
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