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Multiplex Immunofluorescence and Spatial Image Analysis of the Tumor Microenvironment
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Multiplex Immunofluorescence Staining and Image Analysis (Video) | JoVE
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Multiplex Immunofluorescence Staining and Image Analysis

Multiplex Immunofluorescence Staining and Image Analysis (Video) | JoVE

Multiplex Immunofluorescence and Spatial Image Analysis of the Tumor Microenvironment

DOI:
10.3791/200119-v

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04:44 min

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June 02, 2023

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, , , , , , ,
1Institut de Recherche Expérimentale et Clinique (IREC), Pôle MIRO,Université Catholique de Louvain (UCLouvain), 2Institut de Recherche Expérimentale et Clinique (IREC), Pôle de Pneumologie, ORL et Dermatologie (PNEU),Université Catholique de Louvain (UCLouvain), 3Division of Pneumology, Cliniques Universitaires St-Luc,Université Catholique de Louvain (UCLouvain), 4IREC Imaging Platform,Université Catholique de Louvain (UCLouvain), 5Institut Roi Albert II, Department of Medical Oncology and Gastroenterology,Cliniques Universitaires St-Luc

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Multiplex ImmunofluorescenceDeparaffinizationEndogenous Peroxidase InhibitionCitrate BufferMicrowaveTris-buffered SalineTween 20BSAPrimary AntibodyPoly-HRP Secondary AntibodyTyramide ReagentPan-Cytokeratin AntibodyBisBenzimideFluorescent StainingImage Analysis

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