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Analysis of DNA Double-strand Break (DSB) Repair in Mammalian Cells
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Analysis of DNA Double-strand Break (DSB) Repair in Mammalian Cells

Analysis of DNA Double-strand Break (DSB) Repair in Mammalian Cells

DOI:
10.3791/2002-v

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13:10 min

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September 08, 2010

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, ,
1Department of Biology,University of Rochester

Chapters

  • 00:00Title
  • 01:13Introduction
  • 02:22The Reporter Constructs
  • 05:02Integration of the Reporter Constructs and Induction of DSBs
  • 09:18Analysis of the GFP+ and DsRed+ Cells by Flow Cytometry
  • 11:34Representative FACS Results of the DSB Repair Assay
  • 12:31Conclusion

Summary

Automatic Translation

Automatic Translation

This article describes GFP-based fluorescence in vivo assays that separately quantify homologous recombination and nonhomologous end joining in mammalian cells.

Tags

DNA Double-strand BreakRepairMammalian CellsNonhomologous End JoiningNHEJHomologous RecombinationHRPerturbationsPremature AgingTumorigenesisQuantitative MeasurementFluorescent Reporter ConstructsGFP GeneI-SceI EndonucleaseInduction Of DSBsGFP Positive CellsFlow Cytometry

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