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Journal
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Biochemistry
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Measuring Ubiquitylated, SUMOylated, and ADP-Ribosylated DNA-Protein Crosslinks
/
عزل وتطبيع الحمض النووي الذي يحتوي على بروتينات متشابكة
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
Isolation and Normalization of DNA Containing Crosslinked Proteins
Please note that all translations are automatically generated.
Click here for the English version.
عزل وتطبيع الحمض النووي الذي يحتوي على بروتينات متشابكة
Measuring Ubiquitylated, SUMOylated, and ADP-Ribosylated DNA-Protein Crosslinks
DOI:
10.3791/200209-v
•
02:49 min
•
April 21, 2023
•
Yilun Sun
1
Developmental Therapeutics Branch, Center for Cancer Research, National Cancer Institute
,
National Institutes of Health
Tags
DNA
Crosslinked Proteins
Isolation
Normalization
Ubiquitylation
SUMOylation
PBS
DNAzol
Ethanol
Nucleic Acid
Centrifugation
Pellet
DNA Concentration
UV-Vis Spectrometer
Micrococcal Nuclease
Calcium Micrococcal Nuclease Reaction Buffer
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