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Journal
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Bioengineering
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3D Co-Culture Assay: Vascularized Osteogenic Niches for Cancer Drug Screening
/
Quantificando a Formação da Rede Celular Endotelial Após a Co-Cultura 3D de Células Estromais-Endoteliais
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Quantifying the Endothelial Cell Network Formation After the 3D Stromal-Endothelial Cell Co-Culture
Quantificando a Formação da Rede Celular Endotelial Após a Co-Cultura 3D de Células Estromais-Endoteliais
3D Co-Culture Assay: Vascularized Osteogenic Niches for Cancer Drug Screening
DOI:
10.3791/200258-v
•
02:34 min
•
May 19, 2023
•
Lisa A. Krattiger
,
Maria Mitsi
,
Benjamin R. Simona
,
Martin Ehrbar
1
Department of Obstetrics
,
University Hospital Zurich, University of Zurich
,
2
Ectica Technologies AG
Tags
3D Stromal-endothelial Cell Co-culture
GFP Signal
Fluorescence Microscopy
Image Pre-processing
Fiji/ImageJ
Brightness/contrast Adjustment
Median Blur Filter
Image Binning
Angiogenesis Analyzer
Network Quantification
Total Network Length
Number Of Junctions
Isolated Segments
FGF-2
Growth Factors
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