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Journal
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Bioengineering
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3D Co-Culture Assay: Vascularized Osteogenic Niches for Cancer Drug Screening
/
Vurdering af den osteogene differentiering af 3D stromale endotelceller ved overvågning af den alkaliske fosfataseaktivitet
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Assessing the Osteogenic Differentiation of 3D Stromal-Endothelial Cells by Monitoring the Alkaline Phosphatase Activity
Vurdering af den osteogene differentiering af 3D stromale endotelceller ved overvågning af den alkaliske fosfataseaktivitet
3D Co-Culture Assay: Vascularized Osteogenic Niches for Cancer Drug Screening
DOI:
10.3791/200259-v
•
03:44 min
•
May 19, 2023
•
Lisa A. Krattiger
,
Maria Mitsi
,
Benjamin R. Simona
,
Martin Ehrbar
1
Department of Obstetrics
,
University Hospital Zurich, University of Zurich
,
2
Ectica Technologies AG
Tags
3D Stromal-endothelial Cells
Osteogenic Differentiation
Alkaline Phosphatase Activity
HBM-MSCs
GFP-HUVECs
BCIP/NBT Substrate
Cell Lysates
ALP Activity Quantification
DNA Quantification
Mesenchymal Stem Cells
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