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Journal
/
Biology
/
Rat Intestinal Crypts Isolation and Intestinal Organoids Generation
/
Génération de monocouches 2D intestinales de rat à partir d’organoïdes 3D
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Generation of Rat Intestinal 2D Monolayers from 3D Organoids
Please note that all translations are automatically generated.
Click here for the English version.
Génération de monocouches 2D intestinales de rat à partir d’organoïdes 3D
Rat Intestinal Crypts Isolation and Intestinal Organoids Generation
DOI:
10.3791/200274-v
•
02:47 min
•
June 23, 2023
•
Eleanor Zagoren*
1
,
Anderson K. Santos*
1,2
,
Nadia A. Ameen
4
,
Kaelyn Sumigray
5,6
1
Department of Genetics
,
Yale School of Medicine
,
2
Department of Pediatrics
,
Yale School of Medicine
,
3
Department of Pediatrics/Gastroenterology and Hepatology
,
Yale School of Medicine
,
4
Department of Cellular and Molecular Physiology
,
Yale School of Medicine
,
5
Yale Stem Cell Center
,
Yale School of Medicine
,
6
Yale Cancer Center
,
Yale School of Medicine
Tags
Rat Intestinal 2D Monolayers
3D Organoids
EME Coating
Collagen Coating
Organoid Disruption
Trypsin Treatment
RIOM2D
Y27632
2D Monolayers
3D Structures
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