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Sonicação de Formaldeído-Crosslinked
Caenorhabditis elegans
e Imunoprecipitação de Cromatina
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal
Genetics
Sonication of Formaldehyde-Crosslinked
Caenorhabditis elegans
and Chromatin Immunoprecipitation
Sonicação de Formaldeído-Crosslinked
Caenorhabditis elegans
e Imunoprecipitação de Cromatina
DOI:
10.3791/200310-v
•
02:41 min
•
May 05, 2023
•
Chengyin Li*
1
,
Phoebe A. W. Bhagoutie*
1
,
Victor Lao
,
Arneet L. Saltzman
1
Department of Cell and Systems Biology
,
University of Toronto
Tags
Sonication
Formaldehyde-Crosslinked
Caenorhabditis Elegans
Chromatin Immunoprecipitation
Resuspension Buffer
Detergents
Lysate
Centrifugation
Immunoprecipitation
Anti-H3K9 Trimethylation
Anti-histone H3
IgG
Protein G
Magnetic Beads
FA-150 Buffer
Chromatin IP Elution Buffer
Reverse Cross-linking
DNA Elution
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