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Journal
/
Biology
/
A Nanopore-Based Genomic Approach to Control Rabies in Low-Income Countries
/
Monstervoorbereiding, RNA-extractie, PCR-opschoning en kwantificering voor sequencing van het hele genoom van het rabiësvirus (RABV)
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Sample Preparation, RNA Extraction, PCR Clean Up, and Quantification for Whole Genome Sequencing of Rabies Virus (RABV)
Please note that all translations are automatically generated.
Click here for the English version.
Monstervoorbereiding, RNA-extractie, PCR-opschoning en kwantificering voor sequencing van het hele genoom van het rabiësvirus (RABV)
A Nanopore-Based Genomic Approach to Control Rabies in Low-Income Countries
DOI:
10.3791/200317-v
•
03:08 min
•
August 18, 2023
•
Criselda Bautista
2
,
Gurdeep Jaswant
3,4,5
,
Hollie French
6
,
Kathryn Campbell
,
Rowan Durrant
,
Robert Gifford
6
,
Grace S. N. Kia
8
,
Brian Ogoti
9
,
Katie Hampson
,
Kirstyn Brunker
6
1
School of Biodiversity, One Health & Veterinary Medicine
,
University of Glasgow
,
2
Research Institute for Tropical Medicine
,
3
University of Nairobi, Institute of Tropical and Infectious Diseases
,
4
Tanzania Industrial Research Development Organization
,
5
Ifakara Health Institute
,
6
MRC-University of Glasgow Centre for Virus Research
,
7
Department of Veterinary Public health
,
Ahmadu Bello University
,
8
African Centre of Excellence for Neglected Tropical Diseases and Forensic Biotechnology
,
Ahmadu Bello University
,
9
University of Nairobi, Kenya and Center for Epidemiological Modelling and Analysis
,
University of Nairobi
Tags
Keywords: Sample Preparation
RNA Extraction
PCR Clean-up
Quantification
Whole Genome Sequencing
Rabies Virus (RABV)
Ceramic Beads
Lysis Buffer
Brain Sample
CDNA Preparation
PCR Amplification
Primer Pools
SPRI Beads
Magnetic Rack
Ethanol Wash
DNA Product
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