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A Nanopore-Based Genomic Approach to Control Rabies in Low-Income Countries

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Préparation d’échantillons, extraction d’ARN, nettoyage par PCR et quantification pour le séquençage du génome entier du virus de la rage (RABV)

JoVE Journal
Biology

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JoVE Journal Biology

Sample Preparation, RNA Extraction, PCR Clean Up, and Quantification for Whole Genome Sequencing of Rabies Virus (RABV)

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Préparation d’échantillons, extraction d’ARN, nettoyage par PCR et quantification pour le séquençage du génome entier du virus de la rage (RABV)

A Nanopore-Based Genomic Approach to Control Rabies in Low-Income Countries

DOI:

10.3791/200317-v

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03:08 min

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August 18, 2023

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Criselda Bautista², Gurdeep Jaswant^3,4,5, Hollie French⁶, Kathryn Campbell, Rowan Durrant, Robert Gifford⁶, Grace S. N. Kia⁸, Brian Ogoti⁹, Katie Hampson, Kirstyn Brunker⁶

¹School of Biodiversity, One Health & Veterinary Medicine,University of Glasgow, ²Research Institute for Tropical Medicine, ³University of Nairobi, Institute of Tropical and Infectious Diseases, ⁴Tanzania Industrial Research Development Organization, ⁵Ifakara Health Institute, ⁶MRC-University of Glasgow Centre for Virus Research, ⁷Department of Veterinary Public health,Ahmadu Bello University, ⁸African Centre of Excellence for Neglected Tropical Diseases and Forensic Biotechnology,Ahmadu Bello University, ⁹University of Nairobi, Kenya and Center for Epidemiological Modelling and Analysis,University of Nairobi

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Sample Preparation RNA Extraction PCR Clean-up Quantification Whole Genome Sequencing Rabies Virus (RABV)Ceramic Beads Lysis Buffer Brain Sample CDNA Preparation PCR Amplification Primer Pools SPRI Beads Magnetic Rack Ethanol Wash DNA Product

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