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A Nanopore-Based Genomic Approach to Control Rabies in Low-Income Countries

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狂犬病病毒（RABV）基因组的测序、活体和离线碱基检出、分析和解释

JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology

Sequencing, Live and Offline Basecalling, Analysis, and Interpretation of Rabies Virus (RABV) Genome

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狂犬病病毒（RABV）基因组的测序、活体和离线碱基检出、分析和解释

A Nanopore-Based Genomic Approach to Control Rabies in Low-Income Countries

DOI:

10.3791/200318-v

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05:00 min

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August 18, 2023

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Criselda Bautista², Gurdeep Jaswant^3,4,5, Hollie French⁶, Kathryn Campbell, Rowan Durrant, Robert Gifford⁶, Grace S. N. Kia⁸, Brian Ogoti⁹, Katie Hampson, Kirstyn Brunker⁶

¹School of Biodiversity, One Health & Veterinary Medicine,University of Glasgow, ²Research Institute for Tropical Medicine, ³University of Nairobi, Institute of Tropical and Infectious Diseases, ⁴Tanzania Industrial Research Development Organization, ⁵Ifakara Health Institute, ⁶MRC-University of Glasgow Centre for Virus Research, ⁷Department of Veterinary Public health,Ahmadu Bello University, ⁸African Centre of Excellence for Neglected Tropical Diseases and Forensic Biotechnology,Ahmadu Bello University, ⁹University of Nairobi, Kenya and Center for Epidemiological Modelling and Analysis,University of Nairobi

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Sequencing Flow Cell Priming Basecalling Analysis Interpretation Rabies Virus RABV Genome Rampart Arctic RabV MadDog Lineage Assignment Consensus Sequences Mapped Reads Coverage Fastq FastA

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